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Journal / Cancer Research / Isolierung und Erweiterung zytotoxischer…
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JoVE Journal Cancer Research
Isolierung und Erweiterung zytotoxischer Cytokin-induzierter Killer-T-Zellen zur Krebsbehandlung
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Isolierung und Erweiterung zytotoxischer Cytokin-induzierter Killer-T-Zellen zur Krebsbehandlung

Article DOI: 10.3791/60420-v • 11:11 min • January 24th, 2020
January 24th, 2020 •
1,2,3, 4, 4,5, 4,6,7, 1,2, 1,2, 4, 4
1Department of Urology, Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, 5Graduate Institute of Biomedical Sciences, China Medical University, 6Graduate Institute of Immunology, China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center, China Medical University Hospital

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Hier stellen wir ein Protokoll zur Isolierung und Ausdehnung von peripheren mononukleären Blutzellen dar, die von Zytokin-induzierten CD3+CD56+ Killerzellen abgeleitet wurden, und ihre Zytotoxizitätswirkung gegen hämatologische und feste Krebszellen anhand eines in vitro-diagnosebasierten Durchflusszytometriesystems zu veranschaulichen.

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Krebsforschung Ausgabe 155 Adoptivzellimmuntherapie Zytokin-induzierte Killerzelle periphere mononukleäre Blutzellen hämatologische und feste Krebsarten immunzelluläre Therapie zytotoxische Wirkung Durchflusszytometrie
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