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Journal / Immunology and Infection / Analyse van virale SSHHP'S in groep IV met…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Analyse van virale SSHHP'S in groep IV met gebruikmaking van in vitro en silico-methoden
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Analyse van virale SSHHP'S in groep IV met gebruikmaking van in vitro en silico-methoden

Article DOI: 10.3791/60421-v • 10:40 min • December 21st, 2019
December 21st, 2019 •
1, 2, 2
1National Center for Advancing Translational Sciences, National Institutes of Health, 2United States Naval Research Laboratory

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We presenteren een algemeen protocol voor het identificeren van korte stukken van homologe gastheer-pathogen eiwit sequenties (SSHHP'S) ingebed in de virale poly eiwit. SSHHP'S worden herkend door virale proteasen en richten de gerichte vernietiging van specifieke gastheer proteïnen op door verschillende groep IV virussen.

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Immunologie en infectie afgifte 154 enzym Assay FRET niet-structureel eiwit alphavirus groep IV protease SSHHPS gelassay in vitro docking
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