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JoVE Journal
Biochemistry

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用于大规模核相互作用分析的无标签免疫沉淀质谱工作流程
 
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用于大规模核相互作用分析的无标签免疫沉淀质谱工作流程

Article DOI: 10.3791/60432-v
November 17th, 2019

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Summary November 17th, 2019

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Click here for the English version.

描述是一种蛋白质组学工作流程,用于利用感兴趣的特定蛋白质和无标签质谱的免疫亲和力来识别来自核亚细胞部分的蛋白质相互作用伙伴。该工作流程包括亚细胞分馏、免疫沉淀、过滤器辅助样品制备、离线清理、质谱和下游生物信息学管道。

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