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Journal / Biochemistry / Label-fri Immunoprecipitation massespektrometri…
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Biochemistry

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JoVE Journal Biochemistry
Label-fri Immunoprecipitation massespektrometri workflow for storstilet nuklear Interactome profilering
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Label-fri Immunoprecipitation massespektrometri workflow for storstilet nuklear Interactome profilering

Article DOI: 10.3791/60432-v • 11:19 min • November 17th, 2019
November 17th, 2019 •
1, 1, 1,2
1Department of Molecular, Cellular and Developmental Biology, University of Colorado, 2Linda Crnic Institute for Down Syndrome, University of Colorado School of Medicine

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Beskrevet er en proteomics workflow for at identificere protein interaktion partnere fra en nuklear subcellulære fraktion ved hjælp af immun affinitet berigelse af et givet protein af interesse og label-fri massespektrometri. Arbejdsprocessen omfatter subcellulære fraktionering, immunopræcipitation, filter Aided prøveforberedelse, offline oprydning, massespektrometri, og en downstream Bioinformatik pipeline.

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Biokemi massespektrometri proteomics subcellulær fraktionering immunoprecipitation interactome bioinformatik workflow
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