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Journal / Biochemistry / Label-fri immunoprecipitation masspektrometri…
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Biochemistry

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JoVE Journal Biochemistry
Label-fri immunoprecipitation masspektrometri arbetsflöde för storskalig nukleär Interactome profilering
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
Journal (Biochemistry)
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
Journal (Biochemistry)
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
 
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Label-fri immunoprecipitation masspektrometri arbetsflöde för storskalig nukleär Interactome profilering

Article DOI: 10.3791/60432-v • 11:19 min • November 17th, 2019
November 17th, 2019 •
1, 1, 1,2
1Department of Molecular, Cellular and Developmental Biology, University of Colorado, 2Linda Crnic Institute for Down Syndrome, University of Colorado School of Medicine

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Beskrivs är ett proteomik arbetsflöde för att identifiera protein interaktion partner från en nukleär subcellulär fraktion med hjälp av immunoaffinity berikning av ett givet protein av intresse och etikettfri masspektrometri. Arbetsflödet omfattar subcellulär fraktionering, immunoprecipitation, filterstödd provberedning, offlinerensning, masspektrometri och en nedströms bioinformatik-pipeline.

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Biokemi masspektrometri proteomik subcellulär fraktionering immunoprecipitation interactome bioinformatik Workflow
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