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JoVE Journal Neuroscience
Aislamiento y cultivo de las neuronas motoras oculomotoras, trocleas y espinales de Islmnprenatal
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Aislamiento y cultivo de las neuronas motoras oculomotoras, trocleas y espinales de Islmnprenatal :GFP Ratones transgénicos

Article DOI: 10.3791/60440-v • 10:04 min • November 12th, 2019
November 12th, 2019 •
1,2,3,4,9, 1,2,10, 1,2,3,7, 2,5,6, 1,2,3,4,5,6,7,8
1Department of Neurology, Boston Children's Hospital, 2FM Kirby Neurobiology Center, Boston Children's Hospital, 3Department of Neurology, Harvard Medical School, 4Medical Genetics Training Program, Harvard Medical School, 5Department of Ophthalmology, Boston Children's Hospital, 6Department of Ophthalmology, Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology, Kokura Memorial Hospital, 10Department of Genetics, Albert Einstein College of Medicine

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Este trabajo presenta un protocolo para producir cultivos celulares homogéneos de neuronas motoras primarias oculomotoras, trocleas y espinales. Estos cultivos se pueden utilizar para análisis comparativos de las características morfológicas, celulares, moleculares y electrofisiológicas de las neuronas motoras oculares y espinales.

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Neurociencia Número 153 neurona motora neurona oculomotora neurona trochlear cultivo primario cultivo de neuronas motoras embrionarias de ratón FACS IslMN:ratón transgénico GFP purificación celular aislamiento celular
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