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JoVE Journal Neuroscience
Isolement et culture des neurones oculomoteurs, trochlear et moteurs rachidiens de l'islmnprénatal
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Isolement et culture des neurones oculomoteurs, trochlear et moteurs rachidiens de l'islmnprénatal :GFP Souris transgéniques

Article DOI: 10.3791/60440-v • 10:04 min • November 12th, 2019
November 12th, 2019 •
1,2,3,4,9, 1,2,10, 1,2,3,7, 2,5,6, 1,2,3,4,5,6,7,8
1Department of Neurology, Boston Children's Hospital, 2FM Kirby Neurobiology Center, Boston Children's Hospital, 3Department of Neurology, Harvard Medical School, 4Medical Genetics Training Program, Harvard Medical School, 5Department of Ophthalmology, Boston Children's Hospital, 6Department of Ophthalmology, Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology, Kokura Memorial Hospital, 10Department of Genetics, Albert Einstein College of Medicine

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Ce travail présente un protocole pour produire des cultures cellulaires homogènes des neurones moteurs oculomoteurs, trochléaires et spinaux primaires. Ces cultures peuvent être utilisées pour des analyses comparatives des caractéristiques morphologiques, cellulaires, moléculaires et électrophysiologiques des neurones moteurs oculaires et spinaux.

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Neurosciences Numéro 153 neurone moteur neurone oculomotrice neurone trochléaire culture primaire culture embryonnaire de neurones moteurs de souris FACS souris transgénique IslMN:GFP, purification cellulaire isolement cellulaire
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