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Journal / Neuroscience / Isolatie en cultuur van Oculomotor, Trochlear en…
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JoVE Journal Neuroscience
Isolatie en cultuur van Oculomotor, Trochlear en spinale motor neuronen van prenatale ISLMN
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Isolatie en cultuur van Oculomotor, Trochlear en spinale motor neuronen van prenatale ISLMN: GFP transgene muizen

Article DOI: 10.3791/60440-v • 10:04 min • November 12th, 2019
November 12th, 2019 •
1,2,3,4,9, 1,2,10, 1,2,3,7, 2,5,6, 1,2,3,4,5,6,7,8
1Department of Neurology, Boston Children's Hospital, 2FM Kirby Neurobiology Center, Boston Children's Hospital, 3Department of Neurology, Harvard Medical School, 4Medical Genetics Training Program, Harvard Medical School, 5Department of Ophthalmology, Boston Children's Hospital, 6Department of Ophthalmology, Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology, Kokura Memorial Hospital, 10Department of Genetics, Albert Einstein College of Medicine

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Dit werk presenteert een protocol voor het opleveren van homogene celculturen van primaire Oculomotor, trochlear, en ruggengraat motorneuronen. Deze culturen kunnen worden gebruikt voor vergelijkende analyses van de morfologische, cellulaire, moleculaire en elektrofysiologische kenmerken van oculaire en spinale motorneuronen.

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Neurowetenschappen uitgave 153 motorische neuron Oculomotor neuron trochlear neuron primaire cultuur muis embryonale motorische neuron cultuur FACS ISLMN: GFP transgene muis celzuivering celisolatie
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