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Immunology and Infection

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JoVE Journal Immunology and Infection
Um ensaio de carga bacteriana molecular de tuberculose (TB-MBLA)
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Um ensaio de carga bacteriana molecular de tuberculose (TB-MBLA)

Article DOI: 10.3791/60460-v • 10:41 min • April 30th, 2020
April 30th, 2020 •
1, 1,2, 1, 1, 1, 3, 4, 1, 1
1Division of Infection and Global Health, School of Medicine, University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow

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Descrevemos um teste de ensaio de carga bacteriana molecular de tuberculose realizado após a inativação do calor da escória. A inativação do calor torna as amostras de espelo não infecciosas e evita a necessidade de laboratórios de nível de contenção 3 para testes moleculares de tuberculose.

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Imunologia e Infecção Edição 158 tuberculose Mycobacterium tuberculosis viabilidade inativação de calor RNA ensaio de carga bacteriana molecular transcrição reversa quantitativa PCR
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