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Biochemistry

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JoVE Journal Biochemistry
Cellekultur på silicium nitrid membraner og Cryopreparation for Synchrotron røntgen fluorescens Nano-analyse
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Cellekultur på silicium nitrid membraner og Cryopreparation for Synchrotron røntgen fluorescens Nano-analyse

Article DOI: 10.3791/60461-v • 08:26 min • December 10th, 2019
December 10th, 2019 •
1, 1, 2, 2, 1, 2, 1,2
1Inserm, UA7, Synchrotron Radiation for Biomedicine (STROBE), Université Grenoble Alpes, 2ID16A Beamline, ESRF, The European Synchrotron

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Præsenteret her er en protokol for cellekultur på silicium nitrid membraner og springet-frysning forud for røntgenstråler fluorescens Imaging med en Synchrotron kryogen X-ray nanoprobe. Når kun rumtemperatur Nano-analyse er forudsat, de frosne prøver kan yderligere frysetørret. Disse er kritiske skridt til at indhente oplysninger om den intracellulære elementært sammensætning.

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Biokemi kræftceller sporstof synkrotron stråling røntgen Fluorescens mikroskopi kryofiksation frysetørring nanoprobe
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