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Journal / Immunology and Infection / Bestemmelse af immuncelleidentitet og renhed ved…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Bestemmelse af immuncelleidentitet og renhed ved hjælp af epigenetisk baseret kvantitativ PCR
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Bestemmelse af immuncelleidentitet og renhed ved hjælp af epigenetisk baseret kvantitativ PCR

Article DOI: 10.3791/60465-v • 08:02 min • February 19th, 2020
February 19th, 2020 •
1, 1, 1, 2, 1, 3, 3, 3, 1
1Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, 2California Polytechnic State University (Cal Poly), 3Epiontis GmbH, Precision for Medicine

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Her beskriver vi en robust metode til bestemmelse af immuncelleidentitet og renhed gennem epigenetiske signaturer opdaget ved hjælp af kvantitative PCR (qPCR). DNA-demethylering ved et bestemt locus fungerer som en entydig identifikator for en bestemt celletype og giver mulighed for identifikation af CD8+, regulatoriske eller Th17 T-celler.

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Immunologi og infektion qPCR T-celler identitet renhed epigenetik DNA-methylering bisulfitkonvertering celleterapi
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