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JoVE Journal
Cancer Research

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Kultur, Manipulation, og ortotoptransplantation af mus blære Tumor organoider
 
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Kultur, Manipulation, og ortotoptransplantation af mus blære Tumor organoider

Article DOI: 10.3791/60469-v 08:43 min January 31st, 2020
January 31st, 2020

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol indeholder detaljerede eksperimentelle skridt til at etablere en tre-dimensionel in vitro kultur af blæretumor organoider stammer fra kræftfremkaldende-induceret murine blærekræft. Kultur metoder, herunder passaging, genteknologi, og ortotoptransplantation af tumor organoider er beskrevet.

Tags

Kræftforskning tumor organoids 3D-kultur blæretumor invasiv urotelkarcinom lentiviral transduktion ortotopisk transplantation
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