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Journal / Cancer Research / Cultuur, manipulatie en orthotopische…
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Cancer Research

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JoVE Journal Cancer Research
Cultuur, manipulatie en orthotopische transplantatie van mouse bladder tumor organoïden
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Cultuur, manipulatie en orthotopische transplantatie van mouse bladder tumor organoïden

Article DOI: 10.3791/60469-v • 08:43 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1
1Department of Life Sciences, Pohang University of Science and Technology
* These authors contributed equally

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Dit protocol biedt gedetailleerde experimentele stappen om een driedimensionale in vitro cultuur van blaastumororganoïden afgeleid van kankerverwekkende murineblaaskanker vast te stellen. Cultuurmethoden zoals passaging, genetische manipulatie en orthotopische transplantatie van tumororganoïden worden beschreven.

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Kankeronderzoek Kwestie 155 tumororganoïden 3D-cultuur blaastumor invasieve urotheliale carcinoma lentivirale transductie orthotopische transplantatie
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