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Cancer Research

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JoVE Journal Cancer Research
Cultura, Manipulação e Transplante Ortotópico de Organoides tumorais da bexiga de camundongo
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Cultura, Manipulação e Transplante Ortotópico de Organoides tumorais da bexiga de camundongo

Article DOI: 10.3791/60469-v • 08:43 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1
1Department of Life Sciences, Pohang University of Science and Technology
* These authors contributed equally

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Este protocolo fornece medidas experimentais detalhadas para estabelecer uma cultura in vitro tridimensional de organoides tumorais da bexiga derivados do câncer de bexiga induzido pelo cancerígeno. Métodos culturais, incluindo passaging, engenharia genética e transplante ortotópico de organoides tumorais são descritos.

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Pesquisa do Câncer Edição 155 organoides tumorais cultura 3D tumor de bexiga carcinoma urotelolial invasivo transdução lentiviral transplante ortotópico
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