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Journal / Cancer Research / Cultivo, manipulación y trasplante ortotópico de…
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Cancer Research

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JoVE Journal Cancer Research
Cultivo, manipulación y trasplante ortotópico de organoides tumorales de la vejiga de ratón
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Cultivo, manipulación y trasplante ortotópico de organoides tumorales de la vejiga de ratón

Article DOI: 10.3791/60469-v • 08:43 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1
1Department of Life Sciences, Pohang University of Science and Technology
* These authors contributed equally

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Este protocolo proporciona pasos experimentales detallados para establecer un cultivo tridimensional in vitro de organoides tumorales de vejiga derivados del cáncer de vejiga murina inducido por carcinógenos. Se describen métodos de cultivo que incluyen el passaging, la ingeniería genética y el trasplante ortotópico de organoides tumorales.

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Cancer Research Número 155 organoides tumorales cultivo 3D tumor vesical carcinoma urotelial invasivo transducción lentiviral trasplante ortotópico
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