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Developmental Biology

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JoVE Journal Developmental Biology
Ganz-Mount-In-Situ-Hybridisierung in Zebrafisch-Embryonen und Tube-Formations-Assay in iPSC-ECs zur Untersuchung der Rolle von Endoglin in der Gefäßentwicklung
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Ganz-Mount-In-Situ-Hybridisierung in Zebrafisch-Embryonen und Tube-Formations-Assay in iPSC-ECs zur Untersuchung der Rolle von Endoglin in der Gefäßentwicklung

Article DOI: 10.3791/60498-v • 08:27 min • May 28th, 2020
May 28th, 2020 •
*1, *2, 2, 2, 1, 3, 1,2
1Department of Physiology and Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, 2Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 3Wuxi Lung Transplant Center, Wuxi People's Hospital affiliated to Nanjing Medical University
* These authors contributed equally

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Hier wird ein Protokoll zur Ganzmontage-IN-situ-RNA-Hybridisierungsanalyse in Zebrafischen und Rohrbildungs-Assay in patientenabgeleiteten induzierten pluripotenten Stammzell-abgeleiteten Endothelzellen vorgestellt, um die Rolle von Endoglin bei der Gefäßbildung zu untersuchen.

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Entwicklungsbiologie Ausgabe 159 Vollmontage-In-situ-Hybridisierung iPSC-ECs Rohrbildung Gefäßentwicklung HHT Herz-Kreislauf-Erkrankungen
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