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Kvantifisering av proliferative og døde celler i enteroider
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Kvantifisering av proliferative og døde celler i enteroider

Article DOI: 10.3791/60501-v • 10:47 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1, 1, 1, 1, 2, 2,3, 1, 1, 1
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3Department of Pathology, Brigham and Women's Hospital–Harvard Medical School
* These authors contributed equally

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Den presenterte protokollen bruker flyt cytometri for å kvantifisere antall spre og døde celler i kultiverte mus enteroider. Denne metoden er nyttig for å evaluere effekten av medikamentell behandling på organoid spredning og overlevelse.

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Biologi Utgave 155 enteroider tarm flow cytometri spredning EdU propidiumjodid
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