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Cuantificación de células proliferativas y muertas en enteroides
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Cuantificación de células proliferativas y muertas en enteroides

Article DOI: 10.3791/60501-v • 10:47 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1, 1, 1, 1, 2, 2,3, 1, 1, 1
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3Department of Pathology, Brigham and Women's Hospital–Harvard Medical School
* These authors contributed equally

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El protocolo presentado utiliza citometría de flujo para cuantificar el número de células muertas y proliferantes en enteroides de ratón cultivados. Este método es útil para evaluar los efectos del tratamiento farmacológico en la proliferación y supervivencia de los organoides.

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Biología Número 155 enteroides intestino citometría de flujo proliferación EdU yoduro propidium
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