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Kvantifiering av proliferativa och döda celler i Enteroids
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Kvantifiering av proliferativa och döda celler i Enteroids

Article DOI: 10.3791/60501-v • 10:47 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1, 1, 1, 1, 2, 2,3, 1, 1, 1
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3Department of Pathology, Brigham and Women's Hospital–Harvard Medical School
* These authors contributed equally

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Det presenterade protokollet använder flödecytometri för att kvantifiera antalet prolifererande och döda celler i odlade musenteroider. Denna metod är användbar för att utvärdera effekterna av läkemedelsbehandling på organoid spridning och överlevnad.

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Biologi enteroids tarm flödecytometri spridning EdU propiiumjodid
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