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Kvantificering af proliferative og døde celler i Enteroids
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Kvantificering af proliferative og døde celler i Enteroids

Article DOI: 10.3791/60501-v • 10:47 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1, 1, 1, 1, 2, 2,3, 1, 1, 1
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3Department of Pathology, Brigham and Women's Hospital–Harvard Medical School
* These authors contributed equally

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Den præsenterede protokol bruger flow cytometri til at kvantificere antallet af prolifererende og døde celler i dyrkede mus enteroids. Denne metode er nyttig til at vurdere virkningerne af narkotikabehandling på organoid spredning og overlevelse.

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Biologi enteroids tarm flow cytometri spredning EdU propidium iodid
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