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JoVE Journal Developmental Biology
Laser Capture Microdissectie van de muis embryonale kraakbeen en bot voor genexpressie analyse
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Laser Capture Microdissectie van de muis embryonale kraakbeen en bot voor genexpressie analyse

Article DOI: 10.3791/60503-v • 09:20 min • December 18th, 2019
December 18th, 2019 •
1, 1, 1,2, 1, 1
1Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, 2Icahn Institute for Data Science and Genomic Technology, Icahn School of Medicine at Mount Sinai

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Dit protocol beschrijft de laser Capture micro dissectie voor de isolatie van kraakbeen en bot uit verse bevroren delen van de muis embryo. Kraakbeen en botten kunnen snel worden gevisualiseerd door Cresylacetaat-Violet kleuring en precies worden verzameld om RNA van hoge kwaliteit te leveren voor transcriptomische analyse.

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Ontwikkelingsbiologie uitgave 154 laser Capture micro dissectie Meckel van kraakbeen mandibulaire bot Cresylacetaat Violet RNA-isolatie RNA-sequencing
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