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Journal / Immunology and Infection / Immunofluorescenza Colorazione utilizzando IBA1 e …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Immunofluorescenza Colorazione utilizzando IBA1 e TMEM119 per densità microgliale, morfologia e infiltrazione delle cellule mieloidi periferiche nel cervello del mouse
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Immunofluorescenza Colorazione utilizzando IBA1 e TMEM119 per densità microgliale, morfologia e infiltrazione delle cellule mieloidi periferiche nel cervello del mouse

Article DOI: 10.3791/60510-v • 10:40 min • October 27th, 2019
October 27th, 2019 •
1,2, 1,2, 1,3, 1,2,4, 1,2,4, 1,2
1Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, 3Integrated Program in Neuroscience, McGill University, 4Center for Brain Immunology and Glia (BIG), University of Virginia

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Questo protocollo descrive un flusso di lavoro passo-passo per la costaintura immunofluorescente di IBA1 e TMEM119, oltre all'analisi della densità microgliale, della distribuzione e della morfologia, nonché dell'infiltrazione delle cellule mieloidi periferiche nel tessuto cerebrale del topo.

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Immunologia e Infezione Numero 152 Topo cervello microglia cellule mieloidi neuroimmunologia immunofluorescenza IBA1 TMEM119 microscopia
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