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Journal / Immunology and Infection / Imunofluorescência Manchausando IBA1 e TMEM119…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Imunofluorescência Manchausando IBA1 e TMEM119 para densidade microglial, morfologia e análise periférica de infiltração de células mieloides no cérebro do rato
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Imunofluorescência Manchausando IBA1 e TMEM119 para densidade microglial, morfologia e análise periférica de infiltração de células mieloides no cérebro do rato

Article DOI: 10.3791/60510-v • 10:40 min • October 27th, 2019
October 27th, 2019 •
1,2, 1,2, 1,3, 1,2,4, 1,2,4, 1,2
1Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, 3Integrated Program in Neuroscience, McGill University, 4Center for Brain Immunology and Glia (BIG), University of Virginia

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Este protocolo descreve um fluxo de trabalho passo a passo para a costainização imunofluorescente de IBA1 e TMEM119, além da análise da densidade microglial, distribuição e morfologia, bem como infiltração periférica de células mieloides no tecido cerebral do rato.

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Imunologia e Infecção Edição 152 camundongo cérebro microglia células mieloides neuroimunologia imunofluorescência IBA1 TMEM119 microscopia
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