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JoVE Journal Genetics
Detectie van humaan immunodeficiëntie virus type 1 (HIV-1) antisense protein (ASP) RNA transcripten bij patiënten door onderdeel-specifieke RT-PCR
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Detectie van humaan immunodeficiëntie virus type 1 (HIV-1) antisense protein (ASP) RNA transcripten bij patiënten door onderdeel-specifieke RT-PCR

Article DOI: 10.3791/60511-v • 08:01 min • November 27th, 2019
November 27th, 2019 •
1, 1, 2, 3, 4, 5, 2, 1, 1
1Division of Immunology and Allergy, Lausanne University Hospital, 2Department of Fundamental Neuroscience, University of Lausanne, 3Department of Biochemistry, Erasmus Medical Center, 4Theoretical Biology and Biophysics Group, Los Alamos National Laboratories, 5Department of Biochemistry, University of Lausanne

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RNA haarspelden en lussen kunnen fungeren als primers voor reverse transcriptie (RT) bij afwezigheid van sequentie-specifieke primers, interfereren met de studie van overlappende antisense transcripten. We hebben een techniek ontwikkeld die in staat is om streng-specifiek RNA te identificeren, en we hebben het gebruikt om HIV-1 antisense Protein ASP te bestuderen.

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Genetica uitgave 153 ASP antisense-eiwit HIV-1 zelf priming antisense RNA RNA denaturatie RT niet-specifieke priming antisense transcriptie
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