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Immunology and Infection

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JoVE Journal Immunology and Infection
Antibiotika Dereplication bruke antibiotikaresistens plattform
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Antibiotika Dereplication bruke antibiotikaresistens plattform

Article DOI: 10.3791/60536-v • 10:49 min • October 17th, 2019
October 17th, 2019 •
1, 2, 1
1Department of Biochemistry and Biomedical Sciences, M.G. DeGroote Institute for Infectious Disease Research, McMaster University, 2Department of Molecular and Cellular Biology, University of Guelph

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Vi beskriver en plattform som utnytter et bibliotek av isogenic antibiotika resistente Escherichia coli for dereplication av antibiotika. Identiteten til en antibiotika produsert av bakterier eller sopp kan utledes av veksten av E. coli uttrykke sine respektive motstand genet. Denne plattformen er økonomisk effektiv og tidseffektiv.

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Immunologi og infeksjon antibiotika dereplication Drug Discovery aktinomyceter Escherichia coli sekundær metabolitten motstand enzymer naturlig produkt
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