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Journal / Bioengineering / En kvantitativ Fluorescens mikroskopi-baseret…
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Bioengineering

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JoVE Journal Bioengineering
En kvantitativ Fluorescens mikroskopi-baseret enkelt Liposome-analyse til påvisning af den kompositoriske Inhomogenitet mellem individuelle Liposomer
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En kvantitativ Fluorescens mikroskopi-baseret enkelt Liposome-analyse til påvisning af den kompositoriske Inhomogenitet mellem individuelle Liposomer

Article DOI: 10.3791/60538-v • 09:12 min • December 13th, 2019
December 13th, 2019 •
1,3, 1,2,3, 1,2,3
1Center for Nanomedicine and Theranostics, Technical University of Denmark, 2Center for Intestinal Absorption and Transport of Biopharmaceuticals, Technical University of Denmark, 3Department of Health Technology, Technical University of Denmark

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Denne protokol beskriver fremstillingen af Liposomer, og hvordan disse kan være immobiliseret på en overflade og afbildet individuelt i en massiv parallel måde ved hjælp af Fluorescens mikroskopi. Dette giver mulighed for kvantificering af størrelsen og kompositoriske inhomogenitet mellem enkelte Liposomer af befolkningen.

Tags

Bioengineering enkelt Liposomer Fluorescens mikroskopi kompositoriske inhomogenitet Liposom karakterisering membran-protein interaktioner Konfokal mikroskopi
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