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In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

Article DOI: 10.3791/60541-v • 08:08 min • November 1st, 2019
November 1st, 2019 •
1,2, 1,2, 3, 1,2,3
1Heart Research Institute, 2Sydney Medical School, University of Sydney, 3Department of Biomedical Sciences, University of Copenhagen

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Presented here is a protocol to detect macrophage extracellular trap (MET) production in live cell culture using microscopy and fluorescence staining. This protocol can be further extended to examine specific MET protein markers by immunofluorescence staining.

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In Vitro Stimulation Visualization Extracellular Trap Release Differentiated Human Monocyte-derived Macrophages Innate Immunity Inflammatory Pathologies Macrophages Extracellular Trap Mast Release Physiological Growth Primary Cells Human Buffy Coat Preparations Monocyte Differentiation THP-1 Cell Line Immune Cells Isolated Neutrophil Microglia Fragile Extracellular Traps M1 Priming Medium Interferon Gamma Lipopolysaccharide M2 Priming Media Interleukin 4
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