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Immunology and Infection

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JoVE Journal Immunology and Infection
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In vitro stimulatie en visualisatie van extracellulaire val afgifte in gedifferentieerde humane monocyte-afgeleide macrofagen

Article DOI: 10.3791/60541-v • 08:08 min • November 1st, 2019
November 1st, 2019 •
1,2, 1,2, 3, 1,2,3
1Heart Research Institute, 2Sydney Medical School, University of Sydney, 3Department of Biomedical Sciences, University of Copenhagen

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Hier is een protocol voor het opsporen van macrofaag extracellulaire trap (met) productie in levende celcultuur met behulp van microscopie en fluorescentie kleuring. Dit protocol kan verder worden uitgebreid om specifieke MET eiwit markers te onderzoeken door immunofluorescentie kleuring.

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Immunologie en infectie afgifte 153 extracellulaire val macrofaag ONTMOETTE ontsteking SYTOX groen fluorescentiemicroscopie
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