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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro stimulation og visualisering af ekstracellulær fælde frigivelse i differentierede humane monocytter-afledte makrofager
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In vitro stimulation og visualisering af ekstracellulær fælde frigivelse i differentierede humane monocytter-afledte makrofager

Article DOI: 10.3791/60541-v • 08:08 min • November 1st, 2019
November 1st, 2019 •
1,2, 1,2, 3, 1,2,3
1Heart Research Institute, 2Sydney Medical School, University of Sydney, 3Department of Biomedical Sciences, University of Copenhagen

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Præsenteret her er en protokol til påvisning af makrofag ekstrellulær fælde (MET) produktion i levende cellekultur ved hjælp af mikroskopi og fluorescens farvning. Denne protokol kan udvides yderligere til at undersøge specifikke MARKEDSført protein markører ved immunofluorescens farvning.

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Immunologi og infektion ekstracellulær fælde makrofag MØDT betændelse SYTOX grøn Fluorescens mikroskopi
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