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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro stimulering og visualisering av ekstracellulære Trap Release i differensiert Human monocytt-avledet makrofager
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In vitro stimulering og visualisering av ekstracellulære Trap Release i differensiert Human monocytt-avledet makrofager

Article DOI: 10.3791/60541-v • 08:08 min • November 1st, 2019
November 1st, 2019 •
1,2, 1,2, 3, 1,2,3
1Heart Research Institute, 2Sydney Medical School, University of Sydney, 3Department of Biomedical Sciences, University of Copenhagen

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Presentert her er en protokoll for å oppdage macrophage ekstracellulære felle (MET) produksjon i levende cellekultur ved hjelp mikroskopi og fluorescens farging. Denne protokollen kan videre utvides til å undersøke bestemte MET protein markører ved immunofluorescence farging.

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Immunologi og infeksjon ekstracellulære felle macrophage MET betennelse SYTOX grønn fluorescens mikroskopi
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