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Journal / Medicine / Undersöka intestinal barriär nedbrytning i…
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Undersöka intestinal barriär nedbrytning i levande organoider
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Undersöka intestinal barriär nedbrytning i levande organoider

Article DOI: 10.3791/60546-v • 07:18 min • March 26th, 2020
March 26th, 2020 •
1, 2, 1, 1, 2, 3, *1, *1,3
1Division of Molecular and Experimental Surgery, Department of Surgery, Translational Research Center, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 2Department of Medicine 1, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 3Optical Imaging Centre Erlangen (OICE), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg
* These authors contributed equally

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Här beskriver vi en teknik för att kvantifiera barriärintegriteten hos små tarmorganoider. Det faktum att metoden är baserad på levande organoider möjliggör sekventiell undersökning av olika barriärintegritetsmodulerande ämnen eller kombinationer av dessa på ett tidsuppslöjt sätt.

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Medicin inflammatorisk tarmsjukdom interferon gamma tarmorganoider barriärpermeabilitet funktionell analys levande organoidmikroskopi
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