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Journal / Medicine / Undersøgelse intestinal barriere opdeling i…
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Undersøgelse intestinal barriere opdeling i levende organoider
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Undersøgelse intestinal barriere opdeling i levende organoider

Article DOI: 10.3791/60546-v • 07:18 min • March 26th, 2020
March 26th, 2020 •
1, 2, 1, 1, 2, 3, *1, *1,3
1Division of Molecular and Experimental Surgery, Department of Surgery, Translational Research Center, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 2Department of Medicine 1, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 3Optical Imaging Centre Erlangen (OICE), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg
* These authors contributed equally

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Her beskriver vi en teknik til at kvantificere barriereintegriteten af små tarmorganoider. Det forhold, at metoden er baseret på levende organoider, gør det muligt at foretage sekventiel undersøgelse af forskellige barriereintegritetsmodulerende stoffer eller kombinationer heraf på en tidsløst måde.

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Medicin inflammatorisk tarmsygdom interferon gamma tarm organoider barriere permeabilitet funktionel analyse levende organoid mikroskopi
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