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JoVE Journal Developmental Biology
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Article DOI: 10.3791/60551-v • 10:00 min • November 28th, 2019
November 28th, 2019 •
1, 1, 2, 3, 1, 4, 1
1Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología, 2Biotecnología Médica y Farmacéutica CONACYT, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), 3Departamento de Inmunobioquímica, Instituto Nacional de Perinatología, 4Instituto de Neurobiología, UNAM

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Summary
Automatic Translation

This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.

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In Vitro Culture Epithelial Cells Human Amniotic Membrane Anatomical Regions Heterogeneity Functional Properties Protocol Co-amniotic Membrane Umbilical Amnion Placental Amnion Reflected Region Biosafety Cabinet Sterile Conditions Dissecting Forceps Scalpel Saline Solution Chorion/amnion Blood Clots
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