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Immunology and Infection

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JoVE Journal Immunology and Infection
NF-KB-avhengige luciferase aktivisering og kvantifisering av Gene Expression i Salmonella infiserte tissue kultur celler
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NF-KB-avhengige luciferase aktivisering og kvantifisering av Gene Expression i Salmonella infiserte tissue kultur celler

Article DOI: 10.3791/60567-v • 10:57 min • January 12th, 2020
January 12th, 2020 •
1, 1
1Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

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Her presenterer vi en protokoll for å raskt og enkelt måle kjernefysisk faktor Kappa-Light-Chain-Enhancer av aktiverte B-celler (NF-KB) aktivering i cellelinjer uttrykker NF-kB:: luciferase reporter konstruksjoner, via målinger av luminescence i cellen lysat. I tillegg er genuttrykk bestemmes via RT-qPCR isolert fra celler infisert med Salmonella tyfimurium.

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Immunologi og infeksjon NF-KB luciferase luminescence Salmonella vev kultur immunrespons genuttrykk RT-qPCR
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