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Journal / Bioengineering / Generation af transgene rotter ved hjælp af en…
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JoVE Journal Bioengineering
Generation af transgene rotter ved hjælp af en lentiviral Vector Approach
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Generation af transgene rotter ved hjælp af en lentiviral Vector Approach

Article DOI: 10.3791/60570-v • 09:07 min • May 17th, 2020
May 17th, 2020 •
1, 1, 1, 1, 1
1Nencki Institute of Experimental Biology, Polish Academy of Sciences

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Denne artikel har til formål at give metoden for lentiviral transgenese i rotte embryoner ved hjælp af flere injektioner af en virus suspension i zygote perivitelline rummet. Kvindelige rotter, der er parret med en frugtbar mandlig stamme med en anden dominerende pels farve bruges til at generere pseudopregnant plejemødre.

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Tilbagetrækning Transgene rotte lentivirale vektorer perivitelline plads plejemødre
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