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JoVE Journal Developmental Biology
Differenziazione neurale efficiente utilizzando la coltura a cella singola di cellule staminali embrionali umane
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Differenziazione neurale efficiente utilizzando la coltura a cella singola di cellule staminali embrionali umane

Article DOI: 10.3791/60571-v • 11:17 min • January 18th, 2020
January 18th, 2020 •
1, 2, 1
1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, 2NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health

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Presentato qui è un protocollo per la generazione di una coltura unicellulare di cellule staminali embrionali umane e la loro successiva differenziazione in cellule progenitrici neurali. Il protocollo è semplice, robusto, scalabile e adatto per lo screening farmacologico e le applicazioni di medicina rigenerativa.

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Biologia dello sviluppo numero 155 cellule staminali embrionali umane crescita e manutenzione coltura di singole cellule differenziazione cellule progenitrici neurali
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