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JoVE Journal Cancer Research
Identifikation af Transskription Factor Regulators ved hjælp af Medium-Throughput Screening af Arrayed Biblioteker og en Dual-Luciferase-baserede Reporter
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Identifikation af Transskription Factor Regulators ved hjælp af Medium-Throughput Screening af Arrayed Biblioteker og en Dual-Luciferase-baserede Reporter

Article DOI: 10.3791/60582-v • 11:32 min • March 27th, 2020
March 27th, 2020 •
1, 1
1Department of Molecular & Cellular Physiology, Albany Medical College

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For at identificere nye regulatorer af transskription faktorer, vi udviklet en tilgang til at screene arrayed lentiviral eller retroviral RNAi biblioteker ved hjælp af en dual-luciferase-baserede transskriptions-reporter assay. Denne tilgang tilbyder en hurtig og relativt billig måde at screene hundredvis af kandidater i et enkelt eksperiment.

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Kræft forskning transskription faktor arrayed RNAi skærm YAP TAZ TEAD dual-luciferase reporter transskriptionsreporter assay kræft
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