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Immunology and Infection

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JoVE Journal Immunology and Infection
In vivo augmentation av Gut-homing reglerande T-cell induktion
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In vivo augmentation av Gut-homing reglerande T-cell induktion

Article DOI: 10.3791/60585-v • 08:02 min • January 22nd, 2020
January 22nd, 2020 •
1,2, 1, 1, 1,3,4, 1,3
1Division of Regenerative Medicine, Department of Medicine, Loma Linda University, 2Zhengzhou University, 3Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, Long Island University, 4Jinan Infectious Disease Hospital, Shandong University

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Här presenterar vi ett protokoll för in vivo förstoring av Gut-homing reglerande T cell induktion. I detta protokoll är dendritiska celler konstruerade för att lokalt producera höga koncentrationer av den aktiva vitamin D (1,25-dihydroxyvitamin D eller 1,25 [OH]2d) och den aktiva vitamin a (retinoic syra eller ra) de Novo.

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Immunologi och infektion Gut-homing reglerande T-celler 25-hydroxyvitamin D 1 α-hydroxylas cytokrom P450-familjen 27 under familj B medlem 1 1,25-dihydroxyvitamin D FoxP3 dendritiska celler retinaldehydehydrogenas 2 c-c Chemokine receptor typ 9
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