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JoVE Journal Bioengineering
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Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior

Article DOI: 10.3791/60589-v • 10:07 min • January 31st, 2020
January 31st, 2020 •
1, 2, 3, 4,5, 1, 1, 1, 6, 1
1Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, 2School of Mathematics and Statistics, University of Melbourne, 3Institute of Marine Sciences, University of California, Santa Cruz, 4Faculty of Life and Environmental Sciences, University of Tsukuba, 5Microbiology Research Center for Sustainability, University of Tsukuba, 6Department of Ecology and Evolutionary Biology, Princeton University

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Summary
Automatic Translation

A protocol for the generation of dynamic chemical landscapes by photolysis within microfluidic and millifluidic setups is presented. This methodology is suitable to study diverse biological processes, including the motile behavior, nutrient uptake, or adaptation to chemicals of microorganisms, both at the single cell and population level.

Tags

Microbial Behavior Controlled Chemical Landscapes Dynamic Chemical Gradients Microscale Ecology Trade-offs Nutrient Kinetics Population Dynamics Microfluidic Technology Photolysis Chemoattractant Chemotactic Response CAD Software Transparency Film Soft Lithography Clean Room PDMS Mixture Elastomer Curing Agent Vacuum Chamber Pressurized Cleaner
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