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JoVE Journal Genetics
Kombinere Laser Capture Microdissection og Microfluidic qPCR at analysere transskriptionelle profiler af enkelte celler
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Kombinere Laser Capture Microdissection og Microfluidic qPCR at analysere transskriptionelle profiler af enkelte celler: A Systems Biologi tilgang til Opioid Afhængighed

Article DOI: 10.3791/60612-v • 09:54 min • March 8th, 2020
March 8th, 2020 •
1,2, 3, 1, 3, 1
1Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, 2Sidney Kimmel Medical College, Thomas Jefferson University, 3Department of Pharmacology & Physiology, Drexel University College of Medicine

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Denne protokol forklarer, hvordan man indsamler enkelte neuroner, mikroglia, og astrocytter fra den centrale kerne af amygdala med høj nøjagtighed og anatomisk specificitet ved hjælp af laser capture mikrodissection. Derudover forklarer vi vores brug af mikrofluidic RT-qPCR til at måle en delmængde af transskriptionen af disse celler.

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Genetik encellede genekspression laser capture microdissection mikrofluidic qPCR opioid afhængighed anatomisk specificitet amygdala gut mikrobiom glia
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