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Journal / Biochemistry / Kvantitativ analyse av cellulær lipidom eav…
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Biochemistry

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JoVE Journal Biochemistry
Kvantitativ analyse av cellulær lipidom eav saccharomyces cerevisiae ved hjelp av flytende kromatografi kombinert med tandem massespektrometri
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Journal (Biochemistry)
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Kvantitativ analyse av cellulær lipidom eav saccharomyces cerevisiae ved hjelp av flytende kromatografi kombinert med tandem massespektrometri

Article DOI: 10.3791/60616-v • 08:56 min • March 8th, 2020
March 8th, 2020 •
1, 2, 1, 1
1Department of Biology, Concordia University, 2Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry, Concordia University

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Vi presenterer en protokoll ved hjelp av flytende kromatografi kombinert med tandem massespektrometri for å identifisere og kvantifisere store cellulære lipider i Sakkarincerevisiae. Den beskrevne metoden for en kvantitativ vurdering av store lipidklasser i en gjærcelle er allsidig, robust og følsom.

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Biokjemi lipider cellulær lipidom unesterified (fri) fettsyrer glyserfofolipider nøytrale lipider sphingolipider ceramider kardiolipiner lipidutvinning lipidomikk flytende kromatografi massespektrometri
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