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Journal / Cancer Research / Generatie van Orthotopic alvleesklier tumoren en…
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Cancer Research

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JoVE Journal Cancer Research
Generatie van Orthotopic alvleesklier tumoren en ex vivo karakterisering van tumor-Infiltrerende T cel cytotoxiciteit
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Generatie van Orthotopic alvleesklier tumoren en ex vivo karakterisering van tumor-Infiltrerende T cel cytotoxiciteit

Article DOI: 10.3791/60622-v • 06:16 min • December 7th, 2019
December 7th, 2019 •
1,2, 1, 2,3
1Division of Cancer, Department of Surgery and Cancer, Imperial College London, 2Centre for Cancer and Inflammation, Barts Cancer Institute, Queen Mary University of London, 3German Center for Neurodegenerative Diseases (DZNE)

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Dit protocol beschrijft de chirurgische generatie van orthotopic alvleesklier tumoren en de snelle vertering van vers geïsoleerde lymfatische alvleesklier tumoren. Na de spijsvertering kunnen levensvatbare immuuncelpopulaties worden gebruikt voor verdere downstream-analyse, inclusief ex-vivo stimulatie van T-cellen voor intracellulaire cytokine detectie door Flowcytometrie.

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Kankeronderzoek probleem 154 immuun Digest tumor T-cel ex vivo cytokines pancreatic orthotopic KPC Flowcytometrie intracellulaire cytotoxiciteit
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