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Cancer Research

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JoVE Journal Cancer Research
Generation af Ortotopiske tumorer i bugspytkirtlen og ex vivo karakterisering af tumor Infiltrerende T-celle cytotoksicitet
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Generation af Ortotopiske tumorer i bugspytkirtlen og ex vivo karakterisering af tumor Infiltrerende T-celle cytotoksicitet

Article DOI: 10.3791/60622-v • 06:16 min • December 7th, 2019
December 7th, 2019 •
1,2, 1, 2,3
1Division of Cancer, Department of Surgery and Cancer, Imperial College London, 2Centre for Cancer and Inflammation, Barts Cancer Institute, Queen Mary University of London, 3German Center for Neurodegenerative Diseases (DZNE)

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Denne protokol beskriver den kirurgiske generation af ortotopiske pancreastumorer og den hurtige fordøjelse af frisk isolerede murine pancreas tumorer. Efter fordøjelsen kan levedygtige immuncelle populationer anvendes til yderligere downstream-analyse, herunder ex vivo -stimulering af T-celler til intracellulær cytokindetektion ved strømnings cytometri.

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Kræftforskning immun fordøje tumor T-celle ex vivo cytokiner pancreas orthotopic KPC flow cytometri intracellulære cytotoksicitet
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