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JoVE Journal
Developmental Biology

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小鼠胚胎细胞的多路复用单细胞mRNA测序分析
 
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小鼠胚胎细胞的多路复用单细胞mRNA测序分析

Article DOI: 10.3791/60647-v 08:30 min January 7th, 2020
January 7th, 2020

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Summary

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Click here for the English version.

在这里,我们提出了一种多路复用单细胞mRNA测序方法,用于分析小鼠胚胎组织中的基因表达。基于滴滴的单细胞mRNA测序(scRNA-Seq)方法与多路复用策略相结合,可以同时分析来自多个样品的单个细胞,从而显著降低试剂成本并最大限度地减少实验批次效应。

Tags

发育生物学, 问题 155, 单细胞 mRNA 测序, 小鼠胚胎组织, 心脏发育, 多路复用条形码, 去复用, 数据分析
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