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Journal / Biochemistry / PeptiQuick，将膜蛋白一步进生物仿入肽，用于简化蛋白质结合测定…
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Biochemistry
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JoVE Journal Biochemistry
PeptiQuick，将膜蛋白一步进生物仿入肽，用于简化蛋白质结合测定
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Journal (Biochemistry)
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
Journal (Biochemistry)
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PeptiQuick，将膜蛋白一步进生物仿入肽，用于简化蛋白质结合测定

Article DOI: 10.3791/60661-v • 15:04 min • November 2nd, 2019
November 2nd, 2019 •
1, 2, 1
1Department of Biochemistry, University of British Columbia, 2School of Biochemistry, University of Bristol

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我们提出了一种方法，结合膜蛋白纯化和重组到消化片在一个单一的色谱步骤。生物素氨酸脚手架用于通过生物层干涉测量直接表面附着和蛋白质-配体相互作用的测量。

Tags

生物化学， 问题 153， 膜模仿， 纳米光盘， 膜蛋白， 受体， 通道， 运输器， 生物传感器， 脂质， 绞痛素， 生物素-链球蛋白， 蛋白质-蛋白质相互作用
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