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Journal / Biochemistry / PeptiQuick, en enstegs inkorporering av…
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Biochemistry
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JoVE Journal Biochemistry
PeptiQuick, en enstegs inkorporering av membranproteiner i biotinylerade Peptidiscs för strömlinjeformade Proteinbindnings analyser
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PeptiQuick, en enstegs inkorporering av membranproteiner i biotinylerade Peptidiscs för strömlinjeformade Proteinbindnings analyser

Article DOI: 10.3791/60661-v • 15:04 min • November 2nd, 2019
November 2nd, 2019 •
1, 2, 1
1Department of Biochemistry, University of British Columbia, 2School of Biochemistry, University of Bristol

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Vi presenterar en metod som kombinerar membran Proteinrening och beredning till peptidiscs i ett enda kromatografiskt steg. Biotinylerade ställningar används för direkt ytmontering och mätning av proteinligand interaktioner via biolayer interferometri.

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Biokemi membran mimetika nanodisc membranproteiner receptorer kanaler transportörer biosensor lipider colicin biotin-streptavidin protein-protein interaktioner
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