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De CryoAPEX-methode voor elektronenmicroscopieanalyse van membraaneiwitlokalisatie binnen ultrastructureel bewaarde cellen
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De CryoAPEX-methode voor elektronenmicroscopieanalyse van membraaneiwitlokalisatie binnen ultrastructureel bewaarde cellen

Article DOI: 10.3791/60677-v • 11:45 min • February 27th, 2020
February 27th, 2020 •
1, 2, 2,3,4, 1,4,5,6
1Department of Biological Sciences, Purdue University, 2Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 3Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, 4Bindley Biosciences Center, Purdue University, 5Purdue Institute of Integrative Neuroscience, Purdue University, 6Purdue Center for Cancer Research, Purdue University

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Dit protocol beschrijft de cryoAPEX-methode, waarbij een APEX2-gelabeld membraaneiwit kan worden gelokaliseerd door transmissieelektronenmicroscopie binnen een optimaal bewaarde celultrastructuur.

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Biologie CryoAPEX membraaneiwit APEX2 transmissieelektronenmicroscopie cryofixatie hogedrukbevriezing vriessubstitutie
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