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Journal / Medicine / Leisten-Subkutanes weißes Adiposeengewebe (ISWAT) …
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Leisten-Subkutanes weißes Adiposeengewebe (ISWAT) Transplantationsmodell von Murine-Inseln
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Leisten-Subkutanes weißes Adiposeengewebe (ISWAT) Transplantationsmodell von Murine-Inseln

Article DOI: 10.3791/60679-v • 10:14 min • February 16th, 2020
February 16th, 2020 •
*1, *1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 1, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Health Science Center, 2Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, 3School of Pharmaceutical Sciences, Wenzhou Medical University, 4Xenotransplantation Program/Department of Surgery, University of Alabama at Birmingham, 5Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University
* These authors contributed equally

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In diesem Protokoll wird eine Methode der murinen Islet-Isolierung und Transplantation in das inguinale subkutane weiße Fettgewebe beschrieben. Isolierte syngene murinische Inselchen werden mit einem Kellermembranhydrogel in einen murinen Empfänger transplantiert. Der Blutzuckerspiegel der Empfänger wird überwacht und die Histologieanalyse der Islettransplantate durchgeführt.

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Medizin Ausgabe 156 Inseltransplantation murine Inselchen T1DM Transplantationsstelle ISWAT Hydrogel
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