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Modèle de transplantation inguinal Subcutaneous De tissu adipeux blanc (ISWAT) des îlots de Murine
Assessment of Kidney Function in Mouse Models of Glomerular Disease
Journal (Medicine)
Assessment of Kidney Function in Mouse Models of Glomerular Disease
Insertion and Securing the Catheter (Video) | JoVE
Journal (Medicine)
Insertion and Securing the Catheter (Video) | JoVE
 
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Modèle de transplantation inguinal Subcutaneous De tissu adipeux blanc (ISWAT) des îlots de Murine

Article DOI: 10.3791/60679-v • 10:14 min • February 16th, 2020
February 16th, 2020 •
*1, *1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 1, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Health Science Center, 2Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, 3School of Pharmaceutical Sciences, Wenzhou Medical University, 4Xenotransplantation Program/Department of Surgery, University of Alabama at Birmingham, 5Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University
* These authors contributed equally

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Dans ce protocole, une méthode d’isolement et de transplantation d’îlot de murine dans le tissu sous-cutané sous-cutané inguinale d’adipeux est décrite. Des îlots murines syngénéiques isolés sont transplantés dans un récipient de murine utilisant un hydrogel de membrane de sous-sol. Le taux de glucose sanguin des receveurs est surveillé, et l’analyse histologique des greffes d’îcles est effectuée.

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Médecine Numéro 156 transplantation d’îlots îlots murines T1DM site de transplantation ISWAT hydrogel
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