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JoVE Journal
Biochemistry

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通过2',7'-二氯二氢氟辛二乙酸染色检测粘附细胞中的总活性氧物种
 
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通过2',7'-二氯二氢氟辛二乙酸染色检测粘附细胞中的总活性氧物种

Article DOI: 10.3791/60682-v 04:53 min June 23rd, 2020
June 23rd, 2020

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Summary

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在这里,我们提出了一个协议,使用2',7'-二氯二氢氟辛二乙酸酯(DCFH-DA)检测细胞活性氧物种(ROS)。2',7'-二氯二氢氟酸酯(DCFH-DA)。该方法使用荧光显微镜可视化粘附细胞中的细胞ROS定位,并用荧光板读取器量化ROS强度。该协议简单、高效且经济高效。

Tags

生物化学, 第160期, 氧化应激, 2', 7' - 二氯二氢氟酸二乙酸酯, 活性氧种, 粘附细胞, 荧光显微镜, 荧光板读取器
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